|
KMID : 0880220110490040675
|
|
Journal of Microbiology
2011 Volume.49 No. 4 p.675 ~ p.675
|
|
|
A rapid PCR-based approach for molecular identification of filamentous fungi
|
|
Yuanyuan Chen
Zhengxiang Wang
Guiyang Shi
Bernard A. Prior
|
|
|
Abstract
|
|
|
|
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates
|
|
|
KEYWORD
|
|
|
filamentous fungi, genomic DNA extraction, alkaline lysis, internal transcribed spacer regions, identification
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|